UFC 320: Ankalaev vs. Pereira 2 is an upcoming mixed martial arts event produced by the Ultimate Fighting Championship that will take place on October 4, 2025, at the T-Mobile Arena in Paradise, Nevada, part of the Las Vegas Valley, United States.

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This event was originally expected to take place in Guadalajara, Mexico on September 13 and serve as the third annual "Noche UFC" event commemorating Mexico's independence day. However, due to the construction of Arena Guadalajara being stalled, the card was moved to San Antonio, Texas and shifted to a Fight Night. The promotion announced a week later that the numbered event would be moved to October and take place in Las Vegas.

A UFC Light Heavyweight Championship rematch between current champion Magomed Ankalaev and former champion Alex Pereira (also former UFC Middleweight Champion, former Glory Middleweight and Light Heavyweight Champion) is scheduled to headline the event. They previously competed at UFC 313 where Ankalaev defeated Pereira for the championship via unanimous decision.

Former champion (also the inaugural Rizin Light Heavyweight Champion) Ji?í Procházka, who is scheduled to meet former title challenger Khalil Rountree Jr. at the event, is expected to serve as backup and potential replacement for this fight.